TY - JOUR
T1 - The Urinary Microbiome in Women Using Single-Use Versus Reusable Catheters for Intermittent Catheterization
T2 - An Exploratory Substudy of the COMPaRE Trial
AU - van Veen, Felice Emanuela Espèrance
AU - Serkani, Zhaleh Esmi
AU - Berendsen, Sophie
AU - Kraaij, Robert
AU - Bode, Lonneke
AU - Hays, John Philip
AU - Scheepe, Jeroen Ronald
AU - Blok, Bertil Freddo Maarten
N1 - Publisher Copyright:
© 2025 The Author(s). Neurourology and Urodynamics published by Wiley Periodicals LLC.
PY - 2025/9
Y1 - 2025/9
N2 - Aims: To characterize the urinary microbiome in women performing clean intermittent self-catheterization (CISC) and explore microbial changes associated with transitioning from single-use to reusable catheters. Methods: This microbiome study of the COMPaRE trial included female CISC patients with urinary retention randomized to either single-use or reusable catheters. Self-catheterized urine samples were collected at baseline and Week 6 for standard culture and 16S rRNA microbiome analysis. Results: A total of 28 patients (12 reusable, 16 single-use) were included, with a median age of 48 years (IQR 32−60); 67.9% had neurogenic lower urinary tract dysfunction. Escherichia-Shigella (36.3%) and Lactobacillus (22.8%) were the most prevalent genera. PERMANOVA identified significant effects of age (p = 0.003), menopausal status (p < 0.001), and catheterization cause (p = 0.003) on microbiome composition. Transitioning to reusable catheters was associated with significant reductions in Escherichia-Shigella (p < 0.001) and Aerococcus (p < 0.001), and increases in Veillonella (p < 0.001) and Finegoldia (p < 0.001). No significant changes were observed in urine culture results (p = 0.250), alpha diversity as measured by the Shannon index (p = 0.862), or beta diversity as assessed by Bray-Curtis distance (p = 0.096). Conclusions: Escherichia-Shigella and Lactobacillus were the most abundant genera in the urinary microbiome of women performing CISC. Age, menopausal status, and catheterization cause significantly influenced microbiome composition. Although specific microbial shifts were observed following transition to reusable catheters, no significant changes in overall microbial diversity were detected. Larger, well-powered studies are needed to validate these results and assess their potential clinical implications. Trial Registration: Clinical Trial RegistrationNL-OMON54700/NL8296.
AB - Aims: To characterize the urinary microbiome in women performing clean intermittent self-catheterization (CISC) and explore microbial changes associated with transitioning from single-use to reusable catheters. Methods: This microbiome study of the COMPaRE trial included female CISC patients with urinary retention randomized to either single-use or reusable catheters. Self-catheterized urine samples were collected at baseline and Week 6 for standard culture and 16S rRNA microbiome analysis. Results: A total of 28 patients (12 reusable, 16 single-use) were included, with a median age of 48 years (IQR 32−60); 67.9% had neurogenic lower urinary tract dysfunction. Escherichia-Shigella (36.3%) and Lactobacillus (22.8%) were the most prevalent genera. PERMANOVA identified significant effects of age (p = 0.003), menopausal status (p < 0.001), and catheterization cause (p = 0.003) on microbiome composition. Transitioning to reusable catheters was associated with significant reductions in Escherichia-Shigella (p < 0.001) and Aerococcus (p < 0.001), and increases in Veillonella (p < 0.001) and Finegoldia (p < 0.001). No significant changes were observed in urine culture results (p = 0.250), alpha diversity as measured by the Shannon index (p = 0.862), or beta diversity as assessed by Bray-Curtis distance (p = 0.096). Conclusions: Escherichia-Shigella and Lactobacillus were the most abundant genera in the urinary microbiome of women performing CISC. Age, menopausal status, and catheterization cause significantly influenced microbiome composition. Although specific microbial shifts were observed following transition to reusable catheters, no significant changes in overall microbial diversity were detected. Larger, well-powered studies are needed to validate these results and assess their potential clinical implications. Trial Registration: Clinical Trial RegistrationNL-OMON54700/NL8296.
UR - https://www.scopus.com/pages/publications/105011944013
U2 - 10.1002/nau.70119
DO - 10.1002/nau.70119
M3 - Article
C2 - 40708214
AN - SCOPUS:105011944013
SN - 0733-2467
VL - 44
SP - 1474
EP - 1483
JO - Neurourology and Urodynamics
JF - Neurourology and Urodynamics
IS - 7
ER -