The Use of Fibrous, Supramolecular Membranes and Human Tubular Cells for Renal Epithelial Tissue Engineering: Towards a Suitable Membrane for a Bioartificial Kidney

PYW Dankers; JM Boomker; A Huizinga-van de Vlag; Frank Smedts; MC Harmsen; MJA van Luyn

The Use of Fibrous, Supramolecular Membranes and Human Tubular Cells for Renal Epithelial Tissue Engineering: Towards a Suitable Membrane for a Bioartificial Kidney

PYW Dankers, JM Boomker, A Huizinga-van de Vlag, Frank Smedts, MC Harmsen, MJA van Luyn

Pathology

Research output: Contribution to journal › Article › Academic › peer-review

45 Citations (Scopus)

Abstract

A bioartificial kidney, which is composed of a membrane cartridge with renal epithelial cells, can substitute important kidney functions in patients with renal failure. A particular challenge is the maintenance of monolayer integrity and specialized renal epithelial cell functions ex vivo. We hypothesized that this can be improved by electro-spun, supramolecular polymer membranes which show clear benefits in ease of processability. We found that after 7 d, in comparison to conventional microporous membranes, renal tubular cells cultured on top of our fibrous supramolecular membranes formed polarized monolayers, which is prerequisite for a well-functioning bioartificial kidney. In future, these supramolecular membranes allow for incorporation of peptides that may increase cell function even further.

Original language	Undefined/Unknown
Pages (from-to)	1345-1354
Number of pages	10
Journal	Macromolecular Bioscience
Volume	10
Issue number	11
Publication status	Published - 2010

Cite this

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title = "The Use of Fibrous, Supramolecular Membranes and Human Tubular Cells for Renal Epithelial Tissue Engineering: Towards a Suitable Membrane for a Bioartificial Kidney",

abstract = "A bioartificial kidney, which is composed of a membrane cartridge with renal epithelial cells, can substitute important kidney functions in patients with renal failure. A particular challenge is the maintenance of monolayer integrity and specialized renal epithelial cell functions ex vivo. We hypothesized that this can be improved by electro-spun, supramolecular polymer membranes which show clear benefits in ease of processability. We found that after 7 d, in comparison to conventional microporous membranes, renal tubular cells cultured on top of our fibrous supramolecular membranes formed polarized monolayers, which is prerequisite for a well-functioning bioartificial kidney. In future, these supramolecular membranes allow for incorporation of peptides that may increase cell function even further.",

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year = "2010",

language = "Undefined/Unknown",

volume = "10",

pages = "1345--1354",

journal = "Macromolecular Bioscience",

issn = "1616-5187",

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The Use of Fibrous, Supramolecular Membranes and Human Tubular Cells for Renal Epithelial Tissue Engineering: Towards a Suitable Membrane for a Bioartificial Kidney. / Dankers, PYW; Boomker, JM; Huizinga-van de Vlag, A et al.
In: Macromolecular Bioscience, Vol. 10, No. 11, 2010, p. 1345-1354.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - The Use of Fibrous, Supramolecular Membranes and Human Tubular Cells for Renal Epithelial Tissue Engineering: Towards a Suitable Membrane for a Bioartificial Kidney

AU - Dankers, PYW

AU - Boomker, JM

AU - Huizinga-van de Vlag, A

AU - Smedts, Frank

AU - Harmsen, MC

AU - van Luyn, MJA

PY - 2010

Y1 - 2010

N2 - A bioartificial kidney, which is composed of a membrane cartridge with renal epithelial cells, can substitute important kidney functions in patients with renal failure. A particular challenge is the maintenance of monolayer integrity and specialized renal epithelial cell functions ex vivo. We hypothesized that this can be improved by electro-spun, supramolecular polymer membranes which show clear benefits in ease of processability. We found that after 7 d, in comparison to conventional microporous membranes, renal tubular cells cultured on top of our fibrous supramolecular membranes formed polarized monolayers, which is prerequisite for a well-functioning bioartificial kidney. In future, these supramolecular membranes allow for incorporation of peptides that may increase cell function even further.

AB - A bioartificial kidney, which is composed of a membrane cartridge with renal epithelial cells, can substitute important kidney functions in patients with renal failure. A particular challenge is the maintenance of monolayer integrity and specialized renal epithelial cell functions ex vivo. We hypothesized that this can be improved by electro-spun, supramolecular polymer membranes which show clear benefits in ease of processability. We found that after 7 d, in comparison to conventional microporous membranes, renal tubular cells cultured on top of our fibrous supramolecular membranes formed polarized monolayers, which is prerequisite for a well-functioning bioartificial kidney. In future, these supramolecular membranes allow for incorporation of peptides that may increase cell function even further.

M3 - Article

SN - 1616-5187

VL - 10

SP - 1345

EP - 1354

JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

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ER -