Abstract
The aim of this study was to evaluate the suitability of tissue-engineered mucosa (TEM) as a model for studying the acute effects of ionizing radiation (IR) on the oral mucosa. TEM and native non-keratinizing oral mucosa (NNOM) were exposed to a single dose of 16.5 Gy and harvested at 1, 6, 24, 48, and 72 h post-irradiation. DNA damage induced by IR was determined using p53 binding protein 1 (53BP1), and DNA repair was determined using Rad51. Various components of the epithelial layer, basement membrane, and underlying connective tissue were analyzed using immunohistochemistry. The expression of cytokines interleukin-1 beta (IL-1 beta and transforming growth factor beta 1 (TGF-beta 1) was analyzed using an enzyme-linked immunosorbent assay. The expression of DNA damage protein 53BP1 and repair protein Rad51 were increased post-irradiation. The expression of keratin 19, vimentin, collage type IV, desmoglein 3, and integrins alpha 6 and beta 4 was altered post-irradiation. Proliferation significantly decreased at 24, 48, and 72 h post-irradiation in both NNOM and TEM. IR increased the secretion of IL-1 beta, whereas TGF-beta 1 secretion was not altered. All observed IR-induced alterations in TEM were also observed in NNOM. Based on the similar response of TEM and NNOM to IR we consider our TEM construct a suitable model to quantify the acute biological effects of IR.
| Original language | Undefined/Unknown |
|---|---|
| Pages (from-to) | 939-948 |
| Number of pages | 10 |
| Journal | International Journal of Oral and Maxillofacial Surgery |
| Volume | 42 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2013 |
Research programs
- EMC NIHES-01-50-01-A