Transcriptional cdk inhibitors cyc065 and thz1 induce apoptosis in glioma stem cells derived from recurrent gbm

Viktorija Juric, Heiko Düssmann, Martine L.M. Lamfers, Jochen H.M. Prehn, Markus Rehm, Brona M. Murphy*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)
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Abstract

Glioma stem cells (GSCs) are tumour initiating cells which contribute to treatment re-sistance, temozolomide (TMZ) chemotherapy and radiotherapy, in glioblastoma (GBM), the most aggressive adult brain tumour. A major contributor to the uncontrolled tumour cell proliferation in GBM is the hyper activation of cyclin‐dependent kinases (CDKs). Due to resistance to standard of care, GBMs relapse in almost all patients. Targeting GSCs using transcriptional CDK inhibitors, CYC065 and THZ1 is a potential novel treatment to prevent relapse of the tumour. TCGA‐GBM data analysis has shown that the GSC markers, CD133 and CD44 were significantly upregulated in GBM patient tumours compared to non‐tumour tissue. CD133 and CD44 stem cell markers were also expressed in gliomaspheres derived from recurrent GBM tumours. Light Sheet Florescence Microscopy (LSFM) further revealed heterogeneous expression of these GSC markers in glio-maspheres. Gliomaspheres from recurrent tumours were highly sensitive to transcriptional CDK inhibitors, CYC065 and THZ1 and underwent apoptosis while being resistant to TMZ. Apoptotic cell death in GSC subpopulations and non‐stem tumour cells resulted in sphere disruption. Collec-tively, our study highlights the potential of these novel CKIs to induce cell death in GSCs from recurrent tumours, warranting further clinical investigation.

Original languageEnglish
Article number1182
JournalCells
Volume10
Issue number5
DOIs
Publication statusPublished - 12 May 2021

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© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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