Transcriptional profiling reveals progeroid Ercc1(-/Delta) mice as a model system for glomerular aging

B Schermer, V Bartels, P Frommolt, B Habermann, F Braun, JL Schultze, M (Marianne) Roodbergen, Jan Hoeijmakers, Bjorn Schumacher, P Nurnberg, Martijn Dollé, T Benzing, RU Muller, CE Kurschat

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Abstract

Background: Aging-related kidney diseases are a major health concern. Currently, models to study renal aging are lacking. Due to a reduced life-span progeroid models hold the promise to facilitate aging studies and allow examination of tissue-specific changes. Defects in genome maintenance in the Ercc1(-/Delta) progeroid mouse model result in premature aging and typical age-related pathologies. Here, we compared the glomerular transcriptome of young and aged Ercc1-deficient mice to young and aged WT mice in order to establish a novel model for research of aging-related kidney disease. Results: In a principal component analysis, age and genotype emerged as first and second principal components. Hierarchical clustering of all 521 genes differentially regulated between young and old WT and young and old Ercc1(-/Delta) mice showed cluster formation between young WT and Ercc1(-/Delta) as well as old WT and Ercc1(-/Delta) samples. An unexpectedly high number of 77 genes were differentially regulated in both WT and Ercc1(-/Delta) mice (p < 0.0001). GO term enrichment analysis reveal Conclusion: Beyond insulin signaling, we find chemokine and cytokine signaling as well as modifiers of extracellular matrix composition to be subject to major changes in the aging glomerulus. At the level of the transcriptome, the pattern of gene activities is similar in the progeroid Ercc1(-/Delta) mouse model constituting a valuable tool for future studies of aging-associated glomerular pathologies.
Original languageUndefined/Unknown
JournalBMC Genomics
Volume14
DOIs
Publication statusPublished - 2013

Research programs

  • EMC MGC-01-12-03

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