Truncated ETV1, fused to novel tissue-specific genes, and full-length ETV1 in prostate cancer

KGL Hermans, Hetty Korput, Ronald van Marion, Dennis Wijngaart, Angelique van der Made, Natasja Dits, Joost Boormans, Theodorus Kwast, H (Herman) van Dekken, C.H. Bangma, Hanneke Korsten, Robert Kraaij, Guido Jenster, Jan Trapman

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Abstract

In this study, we describe the properties of novel ETV1 fusion genes, encoding N-truncated ETV1 (dETV1), and of full-length ETV1, overexpressed in clinical prostate cancer. We detected overexpression of novel ETV1 fusion genes or of full-length ETV1 in 10% of prostate cancers. Novel ETV1 fusion partners included FOXP1, an EST (EST14), and an endogenous retro,viral repeat sequence (HFRVK17). Like TMPRSS2, EST14 and HERVK17 were prostate-specific and androgen-regulated expressed. This unique expression pattern of most ETV1 fusion partners seems an important determinant in prostate cancer development. In transient reporter assays, full-length ETV1 was a strong transactivator, whereas dETV1 was not. However, several of the biological properties of dETV1 and full-length ETV1 were identical. On stable overexpression, both induced migration and invasion of immortalized non-tumorigenic PNT2C2 prostate epithelial cells. In contrast to dETV1, full-length ETV1 also induced anchorage-independent growth of these cells. PNT2C2 cells stably transfected with dETV1 or full-length ETV1 expression constructs showed small differences in induced expression of target genes. Many genes involved in tumor invasion/metastasis, including uPA/uPAR and MMPs, were up-regulated in both cell types. Integrin beta 3 (ITGB3) was clearly up-regulated by full-length ETV1 but much less by dETV1. Based on the present data and on previous findings, a novel concept of the role of dETV1 and of full-length ETV1 overexpression in prostate cancer is proposed.
Original languageUndefined/Unknown
Pages (from-to)7541-7549
Number of pages9
JournalCancer Research
Volume68
Issue number18
DOIs
Publication statusPublished - 2008

Research programs

  • EMC MM-03-24-01
  • EMC MM-03-49-01

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