Several possible mechanisms for the synergistic anti-tumour effects between tumour necrosis factor alpha (TNF-α) and melphalan after isolated limb perfusion (ILP) have been presented. We found a significant sixfold increase in melphalan tumour tissue concentration after ILP when TNF-α was added to the perfusate, which provides a straightforward explanation for the observed synergism between melphalan and TNF-α in ILP. (C) 2000 Cancer Research Campaign.
Bibliographical noteFunding Information: This work was financed in part by a grant from the Dutch Cancer Society. Boehringer Ingelheim GmbH is acknowledged for generously providing TNF-a.
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