Abstract
DNA typing based on gel electrophoretic separation of DNA fragments, followed by hybridization analysis, has become an important analytical tool in areas ranging from forensic science to population biology. This approach can be extended by combining size separation with sequence-specific separation in denaturing gradient gels; this creates a high resolution two-dimensional pattern. The high information content of this system means that very closely related individuals (even monozygotic twins) can be distinguished and that the genetic events associated with development or cancer, for instance, can be followed. Ultimately, 2-D DNA typing could lead to computerized matching of a single individual's genome to a database of genetic markers.
| Original language | English |
|---|---|
| Pages (from-to) | 336-341 |
| Number of pages | 6 |
| Journal | Trends in Biotechnology |
| Volume | 7 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 1989 |
Bibliographical note
Funding Information:We thank Drs P. Eline Slagboom and E. Mullaart for helpful discussions during the course of this work, part of which was supported by Senetek plc. Locus-specific VNTR probes were kindly provided by Prof. A. J. Jeffreys (University of Leicester, Leicester, UK). We thank Prof. L. S. Lerman (Massachussets Institute of Technology, Cambridge, USA) for the use of his melting program. The probes 33.6 and 33.15 are the subject of patent property and commercial enquiries regarding these probes should be directed to ICI Diagnostics, Gadbrook Park, Rudheath, North-wich, Cheshire CW9 7RA, UK. The two-dimensional DNA typing method is subject to a patent application.
