TY - JOUR
T1 - Type 2 iodothyronine deiodinase transgene expression in the mouse heart causes cardiac-specific thyrotoxicosis
AU - Pachucki, Janusz
AU - Hopkins, James
AU - Peeters, Robin
AU - Tu, Helen
AU - Carvalho, Suzy D.
AU - Kaulbach, Helen
AU - Dale Abel, E.
AU - Wondisford, Frederic E.
AU - Ingwall, Joanne S.
AU - Reed Larsen, P.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - Type 2 iodothyronine deiodinase (D2) catalyzes intracellular 3, 5, 3′ triiodothyronine (T3) production from thyroxine (T4), and its messenger RNA mRNA is highly expressed in human, but not rodent, myocardium. The goal of this study was to identify the effects of D2 expression in the mouse myocardium on cardiac function and gene expression. We prepared transgenic (TG) mice in which human D2 expression was driven by the α-MHC promoter. Despite high myocardial D2 activity, myocardial T3 was, at most, minimally increased in TG myocardium. Although, plasma T3 and T4, growth rate as well as the heart weight was not affected by TG expression, there was a significant increase in heart rate of the isolated perfused hearts, from 284 ± 12 to 350 ± 7 beats/min. This was accompanied by an increase in pacemaker channel (HCN2) but not α-MHC or SERCA II messenger RNA levels. Biochemical studies and 31P-NMR spectroscopy showed significantly lower levels of phosphocreatine and creatine in TG hearts. These results suggest that even mild chronic myocardial thyrotoxicosis, such as may occur in human hyperthyroidism, can cause tachycardia and associated changes in high energy phosphate compounds independent of an increase in SERCA II and α-MHC.
AB - Type 2 iodothyronine deiodinase (D2) catalyzes intracellular 3, 5, 3′ triiodothyronine (T3) production from thyroxine (T4), and its messenger RNA mRNA is highly expressed in human, but not rodent, myocardium. The goal of this study was to identify the effects of D2 expression in the mouse myocardium on cardiac function and gene expression. We prepared transgenic (TG) mice in which human D2 expression was driven by the α-MHC promoter. Despite high myocardial D2 activity, myocardial T3 was, at most, minimally increased in TG myocardium. Although, plasma T3 and T4, growth rate as well as the heart weight was not affected by TG expression, there was a significant increase in heart rate of the isolated perfused hearts, from 284 ± 12 to 350 ± 7 beats/min. This was accompanied by an increase in pacemaker channel (HCN2) but not α-MHC or SERCA II messenger RNA levels. Biochemical studies and 31P-NMR spectroscopy showed significantly lower levels of phosphocreatine and creatine in TG hearts. These results suggest that even mild chronic myocardial thyrotoxicosis, such as may occur in human hyperthyroidism, can cause tachycardia and associated changes in high energy phosphate compounds independent of an increase in SERCA II and α-MHC.
UR - https://www.scopus.com/pages/publications/85047686657
U2 - 10.1210/endo.142.1.7907
DO - 10.1210/endo.142.1.7907
M3 - Article
C2 - 11145561
AN - SCOPUS:85047686657
SN - 0013-7227
VL - 142
SP - 13
EP - 20
JO - Endocrinology
JF - Endocrinology
IS - 1
ER -