Typing and subtyping of Clostridium difficile isolates by using multiple-locus variable-number tandem-repeat analysis

Renate J van den Berg, Inge Schaap, Kate E Templeton, Corné H W Klaassen, Ed J Kuijper

Research output: Contribution to journalArticleAcademicpeer-review

128 Citations (Scopus)

Abstract

Using the genomic sequence of Clostridium difficile strain 630, we developed multiple-locus variable-number tandem-repeat analysis (MLVA) with automated fragment analysis and multicolored capillary electrophoresis as a typing method for C. difficile. All reference strains, representing 31 serogroups, 25 toxinotypes, and 7 known subtypes of PCR ribotype 001, could be discriminated from each other. Application of MLVA to 28 isolates from 7 outbreaks due to the emerging hypervirulent PCR ribotype 027-pulsed-field gel electrophoresis type NAP1 resulted in recognition of 13 clusters. Additionally, 29 toxin A-negative, toxin B-positive isolates belonging to PCR ribotype 017 from eight different countries revealed eight country-specific clusters. MLVA is a highly discriminatory genotyping method and a new tool for subtyping of newly emerging variants of C. difficile.

Original languageEnglish
Pages (from-to)1024-8
Number of pages5
JournalJournal of Clinical Microbiology
Volume45
Issue number3
DOIs
Publication statusPublished - Mar 2007
Externally publishedYes

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