Ultrasonic Characterization of Ibidi μ-Slide I Luer Channel Slides for Studies With Ultrasound Contrast Agents

Reza Pakdaman Zangabad, Hongchen Li, Joop J.P. Kouijzer, Simone A.G. Langeveld, Ines Beekers, Martin Verweij, Nico De Jong, Klazina Kooiman*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)
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Abstract

Understanding and controlling the ultrasound contrast agent&#x2019;s response to an applied ultrasound pressure field is crucial when investigating ultrasound imaging sequences and therapeutic applications. The magnitude and frequency of the applied ultrasonic pressure waves affect the oscillatory response of the ultrasound contrast agent. Therefore, it is important to have an ultrasound compatible and optically transparent chamber in which the acoustic response of the ultrasound contrast agent can be studied. The aim of our study was to determine the <italic>in-situ</italic> ultrasound pressure amplitude in the ibidi &#x03BC;-slide I Luer channel, an optically transparent chamber suitable for cell culture including culture under flow, for all microchannel heights (200, 400, 600, 800 &#x03BC;m). First, the <italic>in-situ</italic> pressure field in the 800 &#x03BC;m-high channel, was experimentally characterized using Brandaris 128 ultra-high-speed camera recordings of microbubbles and a subsequent iterative processing method, upon insonification at 2 MHz, 45&#x00B0; incident angle, and 50 kPa peak negative pressure. Control studies in another cell culture chamber, the CLINIcell, were compared to the obtained results. The pressure amplitude was &#x2013;3.7 dB with respect to the pressure field without the ibidi &#x03BC;-slide. Second, using finite element analysis, we determined the <italic>in-situ</italic> pressure amplitude in the ibidi with the 800 &#x03BC;m channel (33.1 kPa) which was comparable to the experimental value (34 kPa). The simulations were extended to the other ibidi channel heights (200, 400, 600 &#x03BC;m) with either 35&#x00B0; or 45&#x00B0; incident angle, and at 1 MHz and 2 MHz. The predicted <italic>in-situ</italic> ultrasound pressure fields were between -8.7 dB to -1.1 dB of the incident pressure field depending on the listed configurations of ibidi slides with different channel heights, applied ultrasound frequencies, and incident angles. In conclusion, the determined ultrasound <italic>in-situ</italic> pressures demonstrate the acoustic compatibility of the ibidi &#x03BC;-slide I Luer for different channel heights, thereby showing its potential for studying the acoustic behavior of ultrasound contrast agents for imaging and therapy.

Original languageEnglish
Pages (from-to)422-429
Number of pages8
JournalIEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Volume70
Issue number5
DOIs
Publication statusPublished - 1 May 2023

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