Ultrasound contrast agents mediated cell labeling for MRI tracking

Ilya Skachkov; Ying Luan; Sandra Van Tiel; Ton Van Der Steen; Klazina Kooiman; Monique Bernsen; Nico De Jong

doi:10.1109/ULTSYM.2012.0569

Ultrasound contrast agents mediated cell labeling for MRI tracking

Ilya Skachkov, Ying Luan, Sandra Van Tiel, Ton Van Der Steen, Klazina Kooiman, Monique Bernsen, Nico De Jong

Research output: Chapter/Conference proceeding › Conference proceeding › Academic › peer-review

Abstract

In vivo cell tracking is an emerging field and a promising technique for tracking stem cells, metastasis and inflammation. The aim of this study was to find optimal US parameters for ultrasound contrast agent-mediated SPIO-labeling of endothelial cells for MRI tracking. The optimal ultrasound parameters were 40 kPa at 1 MHz, 10,000 cycles, 20 Hz pulse repetition frequency, applied 5 minutes after SPIO addition. This gave a ∼7 times increase of SPIO uptake in endothelial cells in vitro compared to the control with 90% cell viability. This technique could therefore be safely used for labeling of endothelial cells with SPIO for MRI tracking.

Original language	English
Title of host publication	2012 IEEE International Ultrasonics Symposium, IUS 2012
Pages	2278-2281
Number of pages	4
DOIs	https://doi.org/10.1109/ULTSYM.2012.0569
Publication status	Published - 2012
Event	2012 IEEE International Ultrasonics Symposium, IUS 2012 - Dresden, Germany Duration: 7 Oct 2012 → 10 Oct 2012

Publication series

Series	IEEE International Ultrasonics Symposium, IUS
ISSN	1948-5719

Conference

Conference	2012 IEEE International Ultrasonics Symposium, IUS 2012
Country/Territory	Germany
City	Dresden
Period	7/10/12 → 10/10/12

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10.1109/ULTSYM.2012.0569

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title = "Ultrasound contrast agents mediated cell labeling for MRI tracking",

abstract = "In vivo cell tracking is an emerging field and a promising technique for tracking stem cells, metastasis and inflammation. The aim of this study was to find optimal US parameters for ultrasound contrast agent-mediated SPIO-labeling of endothelial cells for MRI tracking. The optimal ultrasound parameters were 40 kPa at 1 MHz, 10,000 cycles, 20 Hz pulse repetition frequency, applied 5 minutes after SPIO addition. This gave a ∼7 times increase of SPIO uptake in endothelial cells in vitro compared to the control with 90% cell viability. This technique could therefore be safely used for labeling of endothelial cells with SPIO for MRI tracking.",

author = "Ilya Skachkov and Ying Luan and {Van Tiel}, Sandra and {Van Der Steen}, Ton and Klazina Kooiman and Monique Bernsen and {De Jong}, Nico",

year = "2012",

doi = "10.1109/ULTSYM.2012.0569",

language = "English",

isbn = "9781467345613",

series = "IEEE International Ultrasonics Symposium, IUS",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "2278--2281",

booktitle = "2012 IEEE International Ultrasonics Symposium, IUS 2012",

note = "2012 IEEE International Ultrasonics Symposium, IUS 2012 ; Conference date: 07-10-2012 Through 10-10-2012",

}

Skachkov, I, Luan, Y, Van Tiel, S , Van Der Steen, T , Kooiman, K , Bernsen, M & De Jong, N 2012, Ultrasound contrast agents mediated cell labeling for MRI tracking. in 2012 IEEE International Ultrasonics Symposium, IUS 2012., 6562427, IEEE International Ultrasonics Symposium, IUS, pp. 2278-2281, 2012 IEEE International Ultrasonics Symposium, IUS 2012, Dresden, Germany, 7/10/12. https://doi.org/10.1109/ULTSYM.2012.0569

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T1 - Ultrasound contrast agents mediated cell labeling for MRI tracking

AU - Skachkov, Ilya

AU - Luan, Ying

AU - Van Tiel, Sandra

AU - Van Der Steen, Ton

AU - Kooiman, Klazina

AU - Bernsen, Monique

AU - De Jong, Nico

PY - 2012

Y1 - 2012

N2 - In vivo cell tracking is an emerging field and a promising technique for tracking stem cells, metastasis and inflammation. The aim of this study was to find optimal US parameters for ultrasound contrast agent-mediated SPIO-labeling of endothelial cells for MRI tracking. The optimal ultrasound parameters were 40 kPa at 1 MHz, 10,000 cycles, 20 Hz pulse repetition frequency, applied 5 minutes after SPIO addition. This gave a ∼7 times increase of SPIO uptake in endothelial cells in vitro compared to the control with 90% cell viability. This technique could therefore be safely used for labeling of endothelial cells with SPIO for MRI tracking.

AB - In vivo cell tracking is an emerging field and a promising technique for tracking stem cells, metastasis and inflammation. The aim of this study was to find optimal US parameters for ultrasound contrast agent-mediated SPIO-labeling of endothelial cells for MRI tracking. The optimal ultrasound parameters were 40 kPa at 1 MHz, 10,000 cycles, 20 Hz pulse repetition frequency, applied 5 minutes after SPIO addition. This gave a ∼7 times increase of SPIO uptake in endothelial cells in vitro compared to the control with 90% cell viability. This technique could therefore be safely used for labeling of endothelial cells with SPIO for MRI tracking.

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U2 - 10.1109/ULTSYM.2012.0569

DO - 10.1109/ULTSYM.2012.0569

M3 - Conference proceeding

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SN - 9781467345613

T3 - IEEE International Ultrasonics Symposium, IUS

SP - 2278

EP - 2281

BT - 2012 IEEE International Ultrasonics Symposium, IUS 2012

T2 - 2012 IEEE International Ultrasonics Symposium, IUS 2012

Y2 - 7 October 2012 through 10 October 2012

ER -

Ultrasound contrast agents mediated cell labeling for MRI tracking

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