Ultrastructural Axon–Myelin Unit Alterations in Multiple Sclerosis Correlate with Inflammation

Aletta M R van den Bosch, Sophie Hümmert, Anna Steyer, Torben Ruhwedel, Jörg Hamann, Joost Smolders, Klaus-Armin Nave, Christine Stadelmann, Maarten H P Kole, Wiebke Möbius, Inge Huitinga

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Abstract

Objective: Changes in the normal-appearing white matter (NAWM) in multiple sclerosis (MS) may contribute to disease progression. Here, we systematically quantified ultrastructural and subcellular characteristics of the axon–myelin unit in MS NAWM and determined how this correlates with low-grade inflammation. Methods: Human brain tissue obtained with short postmortem delay and fixation at autopsy enables systematic quantification of ultrastructural characteristics. In this study, we performed high-resolution immunohis tochemistry and quantitative transmission electron microscopy to study inflammation and ultrastructural characteristics of the axon–myelin unit in MS NAWM (n = 8) and control white matter (WM) in the optic nerve. Results: In the MS NAWM, there were more activated and phagocytic microglia cells (HLA +P2RY12 and Iba1 +CD68 +) and more T cells (CD3 +) compared to control WM, mainly located in the perivascular space. In MS NAWM compared to control WM, there were, as expected, longer paranodes and juxtaparanodes and larger overlap between paranodes and juxtaparanodes. There was less compact myelin wrapping, a lower g-ratio, and a higher frequency of axonal mitochondria. Changes in myelin and axonal mitochondrial frequency correlated positively with the number of active and phagocytic microglia and lymphocytes in the optic nerve. Interpretation: These data suggest that in MS NAWM myelin detachment and uncompact myelin wrapping occurs, potassium channels are unmasked at the nodes of Ranvier, and axonal energy demand is increased, or mitochondrial transport is stagnated, accompanied by increased presence of activated and phagocytic microglia and T cells. These subclinical alterations to the axon–myelin unit in MS NAWM may contribute to disease progression. ANN NEUROL 2023.

Original languageEnglish
JournalAnnals of Neurology
DOIs
Publication statusE-pub ahead of print - 24 Dec 2022

Bibliographical note

Funding for this research was obtained through MS Research grants 17‐975 and 19‐1079 (MoveS), Vici grant 865.17.003 from the Netherlands Research Council, the ERC (MyeliNANO), and the Adelson Medical Research Foundation.

Publisher Copyright:
© 2022 The Authors. Annals of Neurology published by Wiley Periodicals LLC on behalf of American Neurological Association.

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