Validation of the protoporphyrin IX-triplet state lifetime technique for mitochondrial oxygen measurements in the skin

Floor Harms; Sander Bodmer; Harold Raat; Robert jan Stolker; Bert Mik

doi:10.1364/OL.37.002625

Validation of the protoporphyrin IX-triplet state lifetime technique for mitochondrial oxygen measurements in the skin

Floor Harms, Sander Bodmer, Harold Raat, Robert jan Stolker, Bert Mik

Anesthesiology

Research output: Contribution to journal › Article › Academic › peer-review

36 Citations (Scopus)

Abstract

Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that mitochondrial PO2 (mitoPO(2)) can be measured in the skin of a rat after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Calibration of mitoPO(2) measurements was done by comparison with simultaneous measurements of the cutaneous microvascular PO2 This was done under three different conditions: in normal skin tissue, in nonrespiration skin tissue due to the application of cyanide, and in anoxic skin tissue after the ventilation with 100% nitrogen. The results of this study show that it is feasible to measure the mitoPO(2) after the topical application of ALA cream by means of the PpIX-triplet state lifetime technique. (C) 2012 Optical Society of America

Original language	Undefined/Unknown
Pages (from-to)	2625-2627
Number of pages	3
Journal	Optics Letters
Volume	37
Issue number	13
DOIs	https://doi.org/10.1364/OL.37.002625
Publication status	Published - 2012

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10.1364/OL.37.002625

http://hdl.handle.net/1765/74076

Cite this

@article{495f724e6a6945288980d3a6679cc879,

title = "Validation of the protoporphyrin IX-triplet state lifetime technique for mitochondrial oxygen measurements in the skin",

abstract = "Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that mitochondrial PO2 (mitoPO(2)) can be measured in the skin of a rat after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Calibration of mitoPO(2) measurements was done by comparison with simultaneous measurements of the cutaneous microvascular PO2 This was done under three different conditions: in normal skin tissue, in nonrespiration skin tissue due to the application of cyanide, and in anoxic skin tissue after the ventilation with 100% nitrogen. The results of this study show that it is feasible to measure the mitoPO(2) after the topical application of ALA cream by means of the PpIX-triplet state lifetime technique. (C) 2012 Optical Society of America",

author = "Floor Harms and Sander Bodmer and Harold Raat and Stolker, {Robert jan} and Bert Mik",

year = "2012",

doi = "10.1364/OL.37.002625",

language = "Undefined/Unknown",

volume = "37",

pages = "2625--2627",

journal = "Optics Letters",

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publisher = "Optica Publishing Group",

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TY - JOUR

T1 - Validation of the protoporphyrin IX-triplet state lifetime technique for mitochondrial oxygen measurements in the skin

AU - Harms, Floor

AU - Bodmer, Sander

AU - Raat, Harold

AU - Stolker, Robert jan

AU - Mik, Bert

PY - 2012

Y1 - 2012

N2 - Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that mitochondrial PO2 (mitoPO(2)) can be measured in the skin of a rat after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Calibration of mitoPO(2) measurements was done by comparison with simultaneous measurements of the cutaneous microvascular PO2 This was done under three different conditions: in normal skin tissue, in nonrespiration skin tissue due to the application of cyanide, and in anoxic skin tissue after the ventilation with 100% nitrogen. The results of this study show that it is feasible to measure the mitoPO(2) after the topical application of ALA cream by means of the PpIX-triplet state lifetime technique. (C) 2012 Optical Society of America

AB - Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that mitochondrial PO2 (mitoPO(2)) can be measured in the skin of a rat after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Calibration of mitoPO(2) measurements was done by comparison with simultaneous measurements of the cutaneous microvascular PO2 This was done under three different conditions: in normal skin tissue, in nonrespiration skin tissue due to the application of cyanide, and in anoxic skin tissue after the ventilation with 100% nitrogen. The results of this study show that it is feasible to measure the mitoPO(2) after the topical application of ALA cream by means of the PpIX-triplet state lifetime technique. (C) 2012 Optical Society of America

U2 - 10.1364/OL.37.002625

DO - 10.1364/OL.37.002625

M3 - Article

C2 - 22743475

SN - 0146-9592

VL - 37

SP - 2625

EP - 2627

JO - Optics Letters

JF - Optics Letters

IS - 13

ER -