Validation of the protoporphyrin IX-triplet state lifetime technique for mitochondrial oxygen measurements in the skin

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Abstract

Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that mitochondrial PO2 (mitoPO(2)) can be measured in the skin of a rat after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Calibration of mitoPO(2) measurements was done by comparison with simultaneous measurements of the cutaneous microvascular PO2 This was done under three different conditions: in normal skin tissue, in nonrespiration skin tissue due to the application of cyanide, and in anoxic skin tissue after the ventilation with 100% nitrogen. The results of this study show that it is feasible to measure the mitoPO(2) after the topical application of ALA cream by means of the PpIX-triplet state lifetime technique. (C) 2012 Optical Society of America
Original languageUndefined/Unknown
Pages (from-to)2625-2627
Number of pages3
JournalOptics Letters
Volume37
Issue number13
DOIs
Publication statusPublished - 2012

Research programs

  • EMC COEUR-09

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