Virological and serological analysis of a recent Middle East respiratory syndrome coronavirus infection case on a triple combination antiviral regimen

N Spanakis, S Tsiodras, Bart Haagmans, Stalinraj Victor, K Pontikis, A Koutsoukou, NG Koulouris, Ab Osterhaus, Marion Koopmans, A Tsakris

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Abstract

Serological, molecular and phylogenetic analyses of a recently imported case of Middle East respiratory syndrome coronavirus (MERS-CoV) in Greece are reported. Although MERS-CoV remained detectable in the respiratory tract secretions of the patient until the fourth week of illness, viraemia was last detected 2 days after initiation of triple combination therapy with pegylated interferon, ribavirin and lopinavir/ritonavir, administered from Day 13 of illness. Phylogenetic analysis of the virus showed close similarity with other human MERS-CoVs from the recent Jeddah outbreak in Saudi Arabia. Immunoglobulin G (IgG) titres peaked 3 weeks after the onset of illness, whilst IgM levels remained constantly elevated during the follow-up period (second to fifth week of illness). Serological testing confirmed by virus neutralisation assay detected an additional case that was a close contact of the patient. (C) 2014 Published by Elsevier B.V.
Original languageUndefined/Unknown
Pages (from-to)528-532
Number of pages5
JournalInternational Journal of Antimicrobial Agents
Volume44
Issue number6
DOIs
Publication statusPublished - 2014

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