Wako β-D-glucan assay can be used to measure serum β-D-glucan in Sudanese patients to aid with diagnosis of eumycetoma caused by Madurella mycetomatis

Bertrand Nyuykonge, Emmanuel Siddig, Najwa A. Mhmoud, Sahar Bakhiet, Eduard Zijlstra, Annelies Verbon, Ahmed H. Fahal, Wendy W. J. van de Sande*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Web of Science)
3 Downloads (Pure)

Abstract

Background: Eumycetoma is a neglected tropical infection of the subcutaneous tissue commonly caused by the fungus Madurella mycetomatis. Previously, we demonstrated that β-D-glucan was present in the serum of eumycetoma patients. Objective: To compare the performance of the recently approved easy-to-use Wako β-D-glucan assay to that of the Fungitell assay in eumycetoma patients. Methods: Using sera obtained from 41 eumycetoma, 12 actinomycetoma and 29 healthy endemic controls, we measured the β-glucan serum concentrations using the Wako assay and compared the performance to that of the Fungitell assay. Results: With the Fungitell assay, median β-glucan serum concentrations of 208, 70 and 27 pg/ml were obtained for the 41 eumycetoma patients, the 12 actinomycetoma patients and the 29 healthy endemic controls, respectively. With the Wako assay these concentrations were 14.45, 11.57 and 2.5 pg/ml, respectively. We demonstrated that when using the optimized cut-off value (5.5 pg/ml) for the Wako assay, the Wako and Fungitell assays had comparable performance in terms of sensitivity and specificity. Conclusion: The Wako assay is comparable to the Fungitell assay for measurement of serum β-glucan in mycetoma patients and hence can be used in combination with current diagnostic tools. However, this test should be used in combination with other tests to differentiate actinomycetoma from eumycetoma.

Original languageEnglish
Number of pages4
JournalJournal of the European Academy of Dermatology and Venereology
DOIs
Publication statusE-pub ahead of print - 6 Oct 2022

Bibliographical note

Funding Information:
DNDi thanks the Global Health Innovative Technology Fund (GHIT Fund), Japan, for its financial support for this work, and thanks UK aid, UK; Médecins sans Frontières (MSF) International; and the Swiss Agency for Development and Cooperation (SDC), Switzerland for supporting its overall mission.

Publisher Copyright:
© 2022 The Authors. Journal of the European Academy of Dermatology and Venereology published by John Wiley & Sons Ltd on behalf of European Academy of Dermatology and Venereology.

Fingerprint

Dive into the research topics of 'Wako β-D-glucan assay can be used to measure serum β-D-glucan in Sudanese patients to aid with diagnosis of eumycetoma caused by Madurella mycetomatis'. Together they form a unique fingerprint.

Cite this