Water content and water profiles in skin measured by FTIR and Raman spectroscopy

Gerald W. Lucassen*, Peter J. Caspers, Gerwin J. Puppels

*Corresponding author for this work

Research output: Contribution to journalConference articleAcademicpeer-review

12 Citations (Scopus)


We present non-invasive measurements of water content in stratum corneum (SC) by FTIR spectroscopy and water content profiles across the epidermis by Raman microspectroscopy. We apply band fitting to the FTIR spectra to assess changes in SC water content following hydration. While the penetration depth of ATR-FTIR is only few micrometers, the confocal Raman microspectrometer enables successive collection of Raman spectra at a range of depths below skin surface, with an axial resolution of ca. 5μm. Depth resolved water concentration measurements of the epidermis obtained by Raman spectroscopy show clear changes in the water concentration profile as a result of hydration.

Original languageEnglish
Pages (from-to)39-45
Number of pages7
JournalProceedings of SPIE - The International Society for Optical Engineering
Publication statusPublished - 3 Nov 2000
EventComtrolling Tissue Optical Properties: Applications in Clinical Study - Amsterdam, Netherlands
Duration: 5 Jul 20006 Jul 2000


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