Yellow fever virus replicons as an expression system for hepatitis C virus structural proteins

Richard Molenkamp; Engbert A. Kooi; Marjoleine A. Lucassen; Sophie Greve; Joyphi C.P. Thijssen; Willy J.M. Spaan; Peter J. Bredenbeek

doi:10.1128/JVI.77.2.1644-1648.2003

Yellow fever virus replicons as an expression system for hepatitis C virus structural proteins

Richard Molenkamp
, Engbert A. Kooi
, Marjoleine A. Lucassen
, Sophie Greve
, Joyphi C.P. Thijssen
, Willy J.M. Spaan^*
, Peter J. Bredenbeek

^*Corresponding author for this work

Leiden University Medical Centre
Leiden University
Wageningen University & Research

Research output: Contribution to journal › Article › Academic › peer-review

53 Citations (Scopus)

Abstract

Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3′-located conserved sequence 1 and an RNA sequence located in the 5′ end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.

Original language	English
Pages (from-to)	1644-1648
Number of pages	5
Journal	Journal of Virology
Volume	77
Issue number	2
DOIs	https://doi.org/10.1128/JVI.77.2.1644-1648.2003
Publication status	Published - 15 Jan 2003
Externally published	Yes

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10.1128/JVI.77.2.1644-1648.2003

Yellow Fever Virus Replicons as an Expression System for Hepatitis C Virus Structural ProteinsFinal published version, 416 KB

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abstract = "Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3′-located conserved sequence 1 and an RNA sequence located in the 5′ end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.",

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AU - Molenkamp, Richard

AU - Kooi, Engbert A.

AU - Lucassen, Marjoleine A.

AU - Greve, Sophie

AU - Thijssen, Joyphi C.P.

AU - Spaan, Willy J.M.

AU - Bredenbeek, Peter J.

PY - 2003/1/15

Y1 - 2003/1/15

N2 - Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3′-located conserved sequence 1 and an RNA sequence located in the 5′ end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.

AB - Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3′-located conserved sequence 1 and an RNA sequence located in the 5′ end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.

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IS - 2

ER -

Yellow fever virus replicons as an expression system for hepatitis C virus structural proteins

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